The two buses collided around 8:50 a.m. just outside the Lincoln Tunnel at West 41st Street and Dyer Avenue, according to the FDNY and police sources.
Twenty-nine people suffered minor injuries, the FDNY said.
It was not immediately clear how many were taken to the hospital.
The FDNY was continuing to work the scene more than two hours later.
The crash comes about a week after a bus stalled in the tunnel, causing brutal delays during the morning commute.
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